Single gene

MetDETECT™

The MetDETECT™ assay evaluates the MET tyrosine kinase receptor locus and surrounding regions using next-generation sequencing at extremely high coverage to identify mutant alleles for prediction of therapeutic response. The MetDetect™ assay can be applied to both plasma and tumor tissue samples to identify MET amplification that occurs in small percentage of DNA molecules (0.10%).

MetDETECT™ Key Metrics
Regions Analyzed Focal amplification of MET
Sequencing Method Illumina next generation sequencing
Bioinformatics Patented PARE and Digital Karyotyping methods
Assay Sensitivity 100% (95% CI, 48%-100%): detection capability 0.10%
Assay Specificity 100% (95% CI, 85%-100%)
Sequencing Coverage 2,000x
Turn-around Time 3 weeks
Sample Requirements Blood or tumor tissue
Sample Types Plasma or frozen tumor, FFPE, cell lines,
and xenografts
Plasma Sample Input 3-5 ml (minimum 1 ml)
Tumor Sample
Input Required
1 µg (minimum 50 ng)
Multi-Gene

PlasmaSELECT™ 64

PlasmaSELECT™ analyzes circulating tumor DNA for genetic alterations in cancer, eliminating the need for an invasive biopsy or tumor tissue. PlasmaSELECT™ may also be used to evaluate sub-clonal mutations in tumor tissue, when available. PlasmaSELECT™ evaluates a targeted panel of 64 well-characterized cancer genes. Cell-free DNA is extracted from plasma and prepared using proprietary methods that accommodate low abundance sample DNA. Samples are processed using a proprietary capture process and high coverage next-generation sequencing to allow tumor-specific (somatic) mutations, microsatellite instability (MSI) and translocations to be identified with a high sensitivity and specificity. Analyses for sequence mutations or rearrangements can be performed together or separately, depending on the specific alterations of interest.

PlasmaSELECT™ 64 Key Metrics
Regions Analyzed 64 Genes
Sequencing Method Illumina next-generation sequencing
Bioinformatics Patented PARE, Digital Karyotyping and VariantDx
Turn-around Time 2-3 weeks
Sample Requirements Whole blood or plasma
Sample Input Required Two 10 ml tubes of peripheral whole blood; 6-10 ml plasma collected according to PGDx sample preparation instructions (>1-2ml for RUO)