Technology overview

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Technology

Personal Genome Diagnostics offers CancerPRO™ tissue, plasma, and custom panels for PROGENEUSTM that utilize capture-based technology and next generation sequencing to interrogate the most important cancer genes.

Testing for cancer-specific differences:

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Sequence Rearrangements
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Amplifications
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Genomic rearrangement
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Micro-Satellite Instability (MSI)

CancerPRO™ Tissue

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88

This test examines the 88 well-characterized genes in tissue

Download CancerPRO™ Tissue
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Results

CANCERPRO™ panels capture and sequence, at extremely high coverage, the full length of coding exons and selected introns of the genes of interest while utilizing rigorous QC methods and robust and innovative bioinformatics pipelines.

CancerPRO™ reliably identifies somatic genetic alterations in tumors with highsensitivity and specificity, and effectively annotate those alterations to enable focus on the most significant genomic changes.

For Research Use Only. Not for use in diagnostic procedures.

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SAMPLE REQUIREMENTS >50 micron thickness tissue sample; >20% or more of which is tumor; Delivered on 5-10 unstained slides or in FFPE block
Assay Sensitivity >99% for mutations and indels
Assay Specificity (PPV); 5 MAF >99%
Assay Limit of Detection 2% MAF for sequence mutations and indels
Use of Normal Maximizes both sensitivity and specificity; option to run without matched normal is available
Depth of Coverage >1,500x
DNA Amount >1 ug (minimum 50 ng)
These characteristics stand out when compared to other options*
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TEST CHARACTERISTIC CANCERPRO™-Tissue TRUSEQ® AMPLICON ION AMPLISEQ™
Genes Analyzed for Mutations 195 or 76 48 50
Point Mutation Analysis Entire gene
coding sequence
Hotspot only Hotspot only
Genes Analyzed for Copy Number Variation 69 or 14 None None
Genes Analyzed for Rearrangements 24 or 14 None None
Microsatellite Instability Analysis Yes No No
Assay Sensitivity >99% Not reported 98% at 5%
Assay Limit of Detection 2% Not reported 5%
Assay Specificity (PPV);
5% MAF
>99% Not reported Not reported
Assay Specificity (PPV); 2-4% MAF >90% Not reported Not reported
Average Distinct Coverage 500x Not reported Not reported
Use Matched Normal DNA for Best Accuracy and Precision Yes No No
Equipment Required HiSeq® or MiSeq® HiSeq® or MiSeq® Ion Torrent
*Other company product specifications taken from product specification sheets as of 2/1/15; all products are for research use only.
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